Transcriptomics

Dataset Information

7

FoxO RNAi in C2C12 cells


ABSTRACT: C2C12 cells are mouse skeletal muscle cells. These cells were transfected with shRNA against FoxO1, FoxO3, and FoxO4. FoxO1, FoxO3, and FoxO4 are the known paralogues expressed in this cell line. C2C12 cells are transfected with shRNA against FoxO1, FoxO3, and FoxO4, respectively. Colonies were selected for the best depletion of the target FoxOs, respectively. Cells were grown in DMEM medium. Total RNA was extracted from each line. Microarray analysis was performed by following the Affymetrix protocols. The data were analyzed by GCOS system. The target genes that we are interested in were further confirmed by qRT-PCR, reporter assay, and other biochemical and molecular biology assays.

ORGANISM(S): Mus musculus  

SUBMITTER: Robert Tjian   Ji-Hye Pairk  Ping Hu  Ronald A DePinho  Kenneth G Geles 

PROVIDER: E-GEOD-13347 | ArrayExpress | 2008-12-30

SECONDARY ACCESSION(S): GSE13347PRJNA109825

REPOSITORIES: GEO, ArrayExpress

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Publications

Codependent activators direct myoblast-specific MyoD transcription.

Hu Ping P   Geles Kenneth G KG   Paik Ji-Hye JH   DePinho Ronald A RA   Tjian Robert R  

Developmental cell 20081001 4


Although FoxO and Pax proteins represent two important families of transcription factors in determining cell fate, they had not been functionally or physically linked together in mediating regulation of a common target gene during normal cellular transcription programs. Here, we identify MyoD, a key regulator of myogenesis, as a direct target of FoxO3 and Pax3/7 in myoblasts. Our cell-based assays and in vitro studies reveal a tight codependent partnership between FoxO3 and Pax3/7 to coordinatel  ...[more]

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