Transcriptomics

Dataset Information

2

Functional characterization of E- and P-cadherin in invasive breast cancer cells


ABSTRACT: Alterations in the cadherin-catenin adhesion complexes are involved in tumor initiation, progression and metastasis. However, the functional implication of distinct cadherin types in breast cancer biology is still poorly understood. Methods: To compare the functional role of E-cadherin and P-cadherin in invasive breast cancer, we stably transfected these molecules into the MDA-MB-231 cell line, and investigated their effects on motility, invasion and gene expression regulation. Expression of either E- and P-cadherin significantly increased cell aggregation and induced a switch from fibroblastic to epithelial morphology. Although expression of these cadherins did not completely reverse the mesenchymal phenotype of MDA-MB-231 cells, both E- and P-cadherin decreased fibroblast-like migration and invasion through extracellular matrix in a similar way. Moreover, microarray gene expression analysis of MDA-MB-231 cells after expression of E- and P-cadherins revealed that these molecules can activate signaling pathways leading to significant changes in gene expression. Although the expression patterns induced by E- and P-cadherin showed more similarities than differences, 40 genes were differentially modified by the expression of either cadherin type. Microarray gene expression analysis of MDA-MB-231 cells after expression of E- and P-cadherins using different clones for each conditions to reveal that these molecules can activate signaling pathways leading to significant changes in gene expression

ORGANISM(S): Homo sapiens  

SUBMITTER: Amaparo Cano   Gema Moreno-Bueno  Jose Palacios  David Sarrio  Marta Hergueta-Redondo 

PROVIDER: E-GEOD-13362 | ArrayExpress | 2010-06-19

SECONDARY ACCESSION(S): GSE13362PRJNA109777

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2009-04-18 | GSE13362 | GEO
2010-06-24 | E-GEOD-10395 | ArrayExpress
2010-06-24 | E-GEOD-9145 | ArrayExpress
2014-01-25 | E-GEOD-54392 | ArrayExpress
2010-02-22 | GSE14943 | GEO
2010-06-24 | E-GEOD-9147 | ArrayExpress
2010-04-15 | E-GEOD-20435 | ArrayExpress
2010-04-07 | E-GEOD-18833 | ArrayExpress
2015-05-28 | PXD000397 | Pride
2010-03-25 | GSE18833 | GEO