Transcriptomics

Dataset Information

238

High-throughput pyrosequencing of endogenous small RNAs from Arabidopsis thaliana wild-type and ddm1 mutant.


ABSTRACT: DNA methylation is essential for silencing transposable elements and some genes in higher eukaryotes, implying that this modification must be tightly controlled. However, accidental changes in DNA methylation can be transmitted through mitosis, as in cancer, or meiosis, leading to epiallelic variation. Here, we demonstrate the existence of an efficient and faithful mechanism that protects against transgenerational loss of DNA methylation in the plant Arabidopsis. This process is specific to the subset of heavily methylated genomic repeats that are targeted by the RNAi machinery, and does not spread into flanking regions. Remethylation is often progressive over two to four sexual generations. This differential and incremental correction of epigenetic defects may preserve genome stability while increasing adaptive opportunities. 2 samples examined: wild type, and ddm1 mutant.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: C Cruaud   Olivier Voinnet  Martine Boccara  Alexis Sarazin  Manel Esteller  P Wincker  Vincent Colot  J Poulain  Fabiana Heredia  Mario F Fraga  François Roudier  Maria Berdasco  Constance Ciaudo  Felipe K Teixeira 

PROVIDER: E-GEOD-13419 | ArrayExpress | 2009-02-02

SECONDARY ACCESSION(S): GSE13419SRP001257PRJNA110069

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


DNA methylation is essential for silencing transposable elements and some genes in higher eukaryotes, which suggests that this modification must be tightly controlled. However, accidental changes in DNA methylation can be transmitted through mitosis (as in cancer) or meiosis, leading to epiallelic variation. We demonstrated the existence of an efficient mechanism that protects against transgenerational loss of DNA methylation in Arabidopsis. Remethylation is specific to the subset of heavily met  ...[more]

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