Transcriptomics

Dataset Information

0

Transcription profiling of human aging hematopoietic progenitor/stem cells


ABSTRACT: To determine how aging impacts gene expression in hematopoietic stem cells (HSCs), human CD34+ cells from bone marrow (34BM) and mobilized stem cell products (34P38NPBSC) were examined using microarray-based expression profiling. Differential expression changes were confirmed by microarray comparisons of younger and older expanded T-cell populations. Experiment Overall Design: We compared gene expression profiles between younger and older human CD34+ cells from bone marrow (34BM) and CD34+CD38- cells from mobilized stem cell products(34P38NPBSC). We also examined gene expression changes associated with aging in human expanded T-cells. For 34BM and T-cells, biotin-labeling of total RNA (5 µg) was performed as per standard Affymetrix protocol. For the 34P38NPBSC arrays, the single-stranded linear amplification protocol was used to process total RNA (100 nanograms) for the microarrays. Both protocols are described in detail in PMID14706461.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Derek L. Stirewalt   

PROVIDER: E-GEOD-13496 | ArrayExpress | 2009-01-13

SECONDARY ACCESSION(S): GSE13496PRJNA110091

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Similar Datasets

2008-12-01 | GSE13496 | GEO
| GSE67098 | GEO
| GSE99962 | GEO
2003-09-22 | GSE674 | GEO
| GSE37334 | GEO
2007-07-16 | GSE8479 | GEO
2009-12-04 | E-GEOD-16936 | ArrayExpress
2016-05-16 | E-GEOD-78216 | ArrayExpress
2009-12-05 | GSE16936 | GEO
2008-10-25 | E-GEOD-6146 | ArrayExpress