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Myxococcus xanthus cells: wild-type vs. ntrC mutant gene expression during development

ABSTRACT: Transcriptional profiling of Myxococcus xanthus comparing wild-type and mutant gene expression at different developmental time points The mutants in our study, nla4, nla18, nla6 and nla28 are strains that carry a mutation in the corresponding genes nla4 (MXAN2516), nla18 (MXAN3692), nla6(MXAN4042), and nla28(MXAN1167). (MXAN numbers are the conventional gene names; nlas are common names). All four of these genes are response regulators of the ntrC family of transcriptional activators. The mutations were made by insertion of a plasmid (pCR2.1TOPO, invitrogen)into the particular activator gene. Plasmids used carry a region of homology (usually 350-500 bp in length) to the sequence of the activator gene coresponding to the highly conserved central domain that is characteristic of these ntrC family of activators. Plasmids were inserted into the chromosome via homologous recombination at the site of homology to the activator gene. These for genes were originally identified because their mutants have phenotypes with strong defects during development. In all four cases, a mutation in the particular gene gives a phenotype with incomplete or abolished development. In this study, the effects of these mutants were examined on developmental gene expression. Two condition experiment, normal wild-type vs mutant

ORGANISM(S): Myxococcus xanthus  

SUBMITTER: Nora B Caberoy  Anthony Garza    

PROVIDER: E-GEOD-13523 | ArrayExpress| 2014-05-01



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