Transcriptomics

Dataset Information

2

M. catarrhalis Gene Expression in the Chinchilla Nasopharynx


ABSTRACT: M. catarrhalis strain O35E.rpsl was inoculated into the nasopharynx of healthy, male adult chinchillas. 24 hours later the nasopharyngeal tissues were extracted and homogenized. Total RNA was extracted from these tissue samples. Subsequently, M. catarrhalis genome directed primers were utilized to synthesize cDNA from the total RNA sample. As a control, M.catarrhalis strain O35E.rpsl was grown in BHI broth to a Klett density of 200 units and underwent RNA extraction per standard protocols. The genome directed primers mentioned above were utilized to synthesize cDNA. Both cDNA samples were subsequently labelled with either Cy3 or Cy5 and hybridized to a custom Microarrays, Inc. gene chip and scanned after 16 hours. Differential gene expression was measured utilizing the broth grown cells as the baseline and the chinchilla isolated cells as the experimental variable. There are 5 individual sample results included in this series. These represent the data from four individual biological replicates (i.e. 4 different sets of inoculated animals). For each replicate the control samples are simultaneously grown broth samples. Three dye swap experiments were performed.

ORGANISM(S): Moraxella catarrhalis  

SUBMITTER: Eric J Hansen   Todd C Hoopman  Todd C. Hoopman 

PROVIDER: E-GEOD-13559 | ArrayExpress | 2012-04-03

SECONDARY ACCESSION(S): GSE13559PRJNA110745

REPOSITORIES: GEO, ArrayExpress

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Publications

Use of the chinchilla model for nasopharyngeal colonization to study gene expression by Moraxella catarrhalis.

Hoopman Todd C TC   Liu Wei W   Joslin Stephanie N SN   Pybus Christine C   Sedillo Jennifer L JL   Labandeira-Rey Maria M   Laurence Cassie A CA   Wang Wei W   Richardson James A JA   Bakaletz Lauren O LO   Hansen Eric J EJ  

Infection and immunity 20111219 3


Young adult chinchillas were atraumatically inoculated with Moraxella catarrhalis via the nasal route. Detailed histopathologic examination of nasopharyngeal tissues isolated from these M. catarrhalis-infected animals revealed the presence of significant inflammation within the epithelium. Absence of similar histopathologic findings in sham-inoculated animals confirmed that M. catarrhalis was exposed to significant host-derived factors in this environment. Twenty-four hours after inoculation, vi  ...[more]

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