Dataset Information


Somatic embryogenesis

ABSTRACT: Examination of cotyledons after 7, 14, 21 and 28 days on 40mg/L 2,4-D.

ORGANISM(S): Glycine max  

SUBMITTER: Lila O Vodkin   Robin T Shealy  Lila O. Vodkin  Anupama Khanna  Francoise Thibaud-Nissen 

PROVIDER: E-GEOD-136 | ArrayExpress | 2010-06-05



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Clustering of microarray data reveals transcript patterns associated with somatic embryogenesis in soybean.

Thibaud-Nissen Françoise F   Shealy Robin T RT   Khanna Anupama A   Vodkin Lila O LO  

Plant physiology 20030501 1

Globular somatic embryos can be induced from immature cotyledons of soybean (Glycine max L. Merr. cv Jack) placed on high levels of the auxin 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos develop from the adaxial side of the cotyledon, whereas the abaxial side evolves into a callus. Using a 9,280-cDNA clone array, we have compared steady-state RNA from the adaxial side from which embryos develop and from the abaxial callus at five time points over the course of the 4 weeks necessary fo  ...[more]

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