Transcriptomics

Dataset Information

79

Concurrent Versus Individual Binding of HuR and AUF1 to Common Labile Target mRNAs


ABSTRACT: RNA was isolated from material that had been immunoprecipitated (IP) from Hela cells using antibodies recognizing RNA-binding proteins HuR or AUF1, as well as using a control IgG1 antibody. RNA was reverse-transcribed in the presence of [alpha-33P]dCTP and the radiolabeled product used to hybridize human cDNA arrays. The experiment was repeated using three independent sample sets. The samples were numbered HuR-1, HuR-2, HuR-3, AUF1-1, AUF1-2, AUF1-3, IgG1-1, IgG1-2, IgG1-3. HuR represents RNA from IP reactions using an anti-HuR antibody, AUF1 represents RNA from IP reactions using an anti-AUF1 antibody and, IgG1 represents RNA from IP reactions using an anti-IgG1 antibody. The numbers 1, 2 and 3 correspond to the three independent experimental datasets. Keywords = RNa-binding protein Keywords = mRNA stability Keywords = exosome Keywords = polysome Keywords = RNA motif Keywords: ordered

ORGANISM(S): Homo sapiens  

SUBMITTER: Jennifer L Martindale  Xiaoling Yang  Tomoko Kawai   Myriam Gorospe   Krystyna Mazan-Mamczarz   Ashish Lal      

PROVIDER: E-GEOD-1361 | ArrayExpress| 2012-03-27

SECONDARY ACCESSION(S): GSE1361PRJNA90115

REPOSITORIES: GEO, ArrayExpress

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Publications

Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs.

Lal Ashish A   Mazan-Mamczarz Krystyna K   Kawai Tomoko T   Yang Xiaoling X   Martindale Jennifer L JL   Gorospe Myriam M  

The EMBO journal 20040715 15


RNA-binding proteins HuR and AUF1 bind to many common AU-rich target mRNAs and exert opposing influence on target mRNA stability, but the functional interactions between HuR and AUF1 have not been systematically studied. Here, using common target RNAs encoding p21 and cyclin D1, we provide evidence that HuR and AUF1 can bind target transcripts on both distinct, nonoverlapping sites, and on common sites in a competitive fashion. In the nucleus, both proteins were found together within stable ribo  ...[more]

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