Dataset Information


Transcription profiling of human cells stored at ambient temperature in different storage media

ABSTRACT: This series represents the complete series of the human 293h media depleted storage on agarose / rehydration condition course analysis. Samples include Control, monolayer; Control, monolayer/full recovery, antibiotics; Spheroid, no storage; two week storage/0hr recovery; two week storage/full recovery; four week storage/0hr recovery; six week storage/0hr recovery.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Richard F. Helm  

PROVIDER: E-GEOD-1364 | ArrayExpress | 2008-06-11



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Long term metabolic arrest and recovery of HEK293 spheroids involves NF-kappaB signaling and sustained JNK activation.

Jack Graham D GD   Garst James F JF   Cabrera M Carla MC   DeSantis Andrea M AM   Slaughter Stephen M SM   Jervis Jody J   Brooks Andrew I AI   Potts Malcolm M   Helm Richard F RF  

Journal of cellular physiology 20060201 2

Understanding how cells withstand a depletion of intracellular water is relevant to the study of longevity, aging, and quiescence because one consequence of air-drying is metabolic arrest. After removal of medium, HEK293 spheroids with intracellular water content of approximately 65% survived partial vacuum, with antistatic control, for weeks in the dark at 25 degrees C. In contrast, only a limited exposure of monolayers to air was lethal; the mitochondrion being a target of this stress. The pat  ...[more]

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