Transcriptomics

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Identification of candidate genes involved in SOD1G93A glial toxicity


ABSTRACT: To better understand how the expression of a mutant gene that causes ALS can perturb the normal phenotype of astrocytes, and to identify genes that may have a role in their toxic effect on motor neurons, we used oligonucleotide arrays to compare the global gene expression profiles of glia overexpressing the mutant SOD1G93A protein with two different sets of controls: non-transgenic glia and glia overexpressing the wild type form of the human SOD1 protein (P<0.001) . Glia were derived from P1-P3 mouse pups transgenic for SOD1G93A (G93A), SOD1WT (WT) or non-transgenic pups (NT). Once the cells reached confluence, total RNA was isolated using Trizol (Invitrogen) from three different biological replicates for each type of glia. RNA was amplified by one round of T7 transcription using the Illumina TotalPrep RNA Amplification Kit. Illumina Bead Array Reader. Analysis was done using the Illumina Bead Studio Program.

ORGANISM(S): Mus musculus  

SUBMITTER: Francesco P Di Giorgio   Gabriella L Boulting  Gabriella Lutz Boulting  Samuel Bobrowicz  Kevin C Eggan 

PROVIDER: E-GEOD-13643 | ArrayExpress | 2010-06-24

SECONDARY ACCESSION(S): GSE13643PRJNA110193

REPOSITORIES: GEO, ArrayExpress

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Publications

Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation.

Di Giorgio Francesco Paolo FP   Boulting Gabriella L GL   Bobrowicz Samuel S   Eggan Kevin C KC  

Cell stem cell 20081201 6


It has been proposed that human embryonic stem cells could be used to provide an inexhaustible supply of differentiated cell types for the study of disease processes. Although methods for differentiating embryonic stem cells into specific cell types have become increasingly sophisticated, the utility of the resulting cells for modeling disease has not been determined. We have asked whether specific neuronal subtypes produced from human embryonic stem cells can be used to investigate the mechanis  ...[more]

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