Transcriptomics

Dataset Information

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Transcriptional profiling of p56lck-deficient regulatory and memory T cells


ABSTRACT: Signaling through the T cell antigen receptor is essential for the formation of regulatory T (Treg) cells in the thymus and for their involvement in antigen-directed suppression of immune responses. Using a conditional null allele of the gene encoding p56Lck we show here that T cell antigen receptor (TCR) signaling is also essential for sustaining the phenotype and homeostasis of Treg cells. Inactivation of p56Lck in Treg cells resulted in large-scale changes in their gene expression profile, blocked their capacity to suppress responses, inhibited their proliferation, and caused them to redistribute in the body. The results make clear multiple aspects of the Treg cell phenotype that are dependent on a sustained capacity to respond through their TCRs. Keywords: Genetic deficiency of p56lck Two-condition experiment: wild-type memory or regulatory T cells versus lck-deficient memory or regulatory T cells.

ORGANISM(S): Mus musculus  

SUBMITTER: Nigel Killeen   Mark Klinger 

PROVIDER: E-GEOD-13645 | ArrayExpress | 2010-05-22

SECONDARY ACCESSION(S): GSE13645PRJNA110195

REPOSITORIES: GEO, ArrayExpress

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Publications

Impact of the TCR signal on regulatory T cell homeostasis, function, and trafficking.

Kim Joong Kyu JK   Klinger Mark M   Benjamin Jonathan J   Xiao Yuanyuan Y   Erle David J DJ   Littman Dan R DR   Killeen Nigel N  

PloS one 20090811 8


Signaling through the T cell antigen receptor (TCR) is important for the homeostasis of naïve and memory CD4(+) T cells. The significance of TCR signaling in regulatory T (Treg) cells has not been systematically addressed. Using an Ox40-cre allele that is prominently expressed in Treg cells, and a conditional null allele of the gene encoding p56(Lck), we have examined the importance of TCR signaling in Treg cells. Inactivation of p56(Lck) resulted in abnormal Treg homeostasis characterized by im  ...[more]

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