Dataset Information


5448 vs 5448R- in vivo 24h

ABSTRACT: Microarray analysis was performed on in vivo-derived RNA from the well characterized GAS strain 5448 and its derivative 5448R-, containing the mutated ropB allele from 5628. We followed a cyclic design that allowed us to compare every condition to each other at least twice, and guaranteed dye swapping to eliminate effects of non-specific binding. This study consists of 4 replicates of 5448 and 3 replicates of 5448R-.

ORGANISM(S): Streptococcus pyogenes  

SUBMITTER: Rita Kansal   Victor Nizet  Mark J Walker  Andrew Hollands  Malak Kotb  Ramy K Aziz 

PROVIDER: E-GEOD-13656 | ArrayExpress | 2010-05-15



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A naturally occurring mutation in ropB suppresses SpeB expression and reduces M1T1 group A streptococcal systemic virulence.

Hollands Andrew A   Aziz Ramy K RK   Kansal Rita R   Kotb Malak M   Nizet Victor V   Walker Mark J MJ  

PloS one 20081231 12

Epidemiological studies of group A streptococcus (GAS) have noted an inverse relationship between SpeB expression and invasive disease. However, the role of SpeB in the course of infection is still unclear. In this study we utilize a SpeB-negative M1T1 clinical isolate, 5628, with a naturally occurring mutation in the gene encoding the regulator RopB, to elucidate the role of RopB and SpeB in systemic virulence. Allelic exchange mutagenesis was used to replace the mutated ropB allele in 5628 wit  ...[more]

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