Transcriptomics

Dataset Information

4

Transcription profiling of normal mouse myeloid cell populations


ABSTRACT: Normal myeloid lineage cell populations (C57BL/6 mice, aged 4-10 weeks, male or female) with three distinct immunophenotypes were prospectively isolated and characterized. In preparation for FACS sorting, bone marrow cells were separated into c-kit+ and c-kit- fractions using an AutoMACS device. C-kit+ cells were further fractionated based on Gr1 and Mac1 expression, and absence of lineage antigen expression (B220, TER119, CD3, CD4, CD8 and IL7Rα), by cell sorting. C-kit+ Gr1+ Mac1lo/- and c-kit+ Gr1+ Mac1+ displayed cytologic features of undifferentiated hematopoietic cells or myeloblasts, whereas c-kit- Gr1+ Mac1+ cells were mature neutrophils. Experiment Overall Design: See summary.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Mus musculus  

SUBMITTER: Tim C. Somervaille  

PROVIDER: E-GEOD-13693 | ArrayExpress | 2009-02-21

SECONDARY ACCESSION(S): GSE13693PRJNA114581

REPOSITORIES: GEO, ArrayExpress

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