Transcriptomics

Dataset Information

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DNA methylation in B cells


ABSTRACT: Using a methylated-DNA enrichment technique (MIRA) in combination with whole genome tiling arrays, we have characterized by MIRA-chip the entire B cell ‘methylome’ of an individual human at 100 base pair resolution. Methylated fragments in genomic DNA extracted from B cells were enriched with the MIRA assay and hybridized together with input genomic DNA to NimbleGen's whole genomc tiling array.

ORGANISM(S): Homo sapiens  

SUBMITTER: Xueyan Zhong   Xiwei Wu  Gerd P Pfeifer  Arthur D Riggs  Tibor Rauch 

PROVIDER: E-GEOD-13700 | ArrayExpress | 2008-12-31

SECONDARY ACCESSION(S): GSE13700PRJNA109427

REPOSITORIES: GEO, ArrayExpress

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Publications

A human B cell methylome at 100-base pair resolution.

Rauch Tibor A TA   Wu Xiwei X   Zhong Xueyan X   Riggs Arthur D AD   Pfeifer Gerd P GP  

Proceedings of the National Academy of Sciences of the United States of America 20090112 3


Using a methylated-DNA enrichment technique (methylated CpG island recovery assay, MIRA) in combination with whole-genome tiling arrays, we have characterized by MIRA-chip the entire B cell "methylome" of an individual human at 100-bp resolution. We find that at the chromosome level high CpG methylation density is correlated with subtelomeric regions and Giemsa-light bands (R bands). The majority of the most highly methylated regions that could be identified on the tiling arrays were associated  ...[more]

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