Transcriptomics

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AHR mediated gene expression changes in immature DN thymocytes and thymic emigrants


ABSTRACT: Effect of an immunosupressive dose of TCDD, a ligand for the aryl hydrocarbon receptor, on the gene expression profile of fetal DN thymocytes and thymic emigrants C57BL6 mice were mated and the pregnant dams sacrifice at day 15 of gestation. Fetal thymic lobes were prepared and cultured for 6 days on culture inserts (5-6 lobes/culture insert). Medium, containing either 10 nM TCDD or solvent control was exchanged every 48 hours. At day six (gestation day 21, corresponding to birth), single cell suspensions of the thymocytes were prepared and CD4-CD8- cells isolated by MACS sorting. Purity of the population was checked FACS analysis, to be greater 95%. RNA was purified (TRIZOL), amplified (Ambion Kit) and processed (Affymetrix standard protocol) according to manufacturers instructions. Raw cell files were processed with the bioconductor affy package

ORGANISM(S): Mus musculus  

SUBMITTER: Marc Majora   Charlotte Esser  Markus Frericks 

PROVIDER: E-GEOD-13720 | ArrayExpress | 2008-11-25

SECONDARY ACCESSION(S): GSE13720PRJNA110399

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptional signatures of immune cells in aryl hydrocarbon receptor (AHR)-proficient and AHR-deficient mice.

Frericks Markus M   Temchura Vladimir V VV   Majora Marc M   Stutte Susanne S   Esser Charlotte C  

Biological chemistry 20060901 9


The ligand-activated aryl hydrocarbon receptor (AHR) is known to modulate many genes in a highly cell-specific manner, either directly or indirectly via secondary effects. In contrast, little is known about the effects of AHR deficiency on gene expression balance. We compared the transcriptome of CD4 T cells from AHR-/- mice and wild-type mice; 390 genes, many of them immunotypic, were deregulated in AHR-deficient CD4 cells. TCDD-induced transcriptome changes correlated with the AHR expression l  ...[more]

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