Transcriptomics

Dataset Information

9

Comparison of naïve, postmitotic (CD44-lo/CD62L-hi) and effector (CD44-hi/CD62L-lo) CD8+ Tcells during BMT in GVHD mice


ABSTRACT: Naïve CD44-lo/CD62L-hi/CD8+ T cells from C3H.SW mice were compared to CD44-hi/CD82L-lo/CD8+ effector memory T cells and CD44-lo/CD62L-hi/CD8+ postmitotic T cells, using 3 biological replicates of each type of sample. The later two cells types were highly purified at day 14 after transplantation from GVHD B6/SJL mice receiving donor C3H.SW mouse-derived naive CD44-lo/CD62L-hi/CD8+ T cells and T cell-depleted bone marrow. Recipient mice had first been lethally irradiated at a dose of 10Gy in two fractions. This is a MHC-identical minor histocompatibility antigen-mismatched mouse GVHD model of human allogeneic hematopoietic stem cell transplantation. Naive T cell samples were from pools of 2 mice each, while effector memory and postmitotic T cell samples were purified from pools of T cells from 4 mice each. After RNA extraction and cleanup, biotin labeled cRNA was prepared from 600 ng total RNA, using two rounds of in vitro transcription, and hybridized to Affymetrix Mouse Genome 430A 2.0 arrays using standard techniques. Keywords: Cell type comparison 9 samples were analyzed on 9 Affymetrix microarrays to assay mRNA levels. There were 3 biological replicates of each of 3 different cell types.

ORGANISM(S): Mus musculus  

SUBMITTER: James L Ferrara  Elizabeth Hexner   Peter Klein   Yi Zhang   Jiu Jiang   Shin Mineishi   Shuaiyang Cui   Jian Huang   Jina Wang   Elizabeth Bacon   Rork Kuick   Koji Kato   Stephan G Emerson   Pavan Reddy    

PROVIDER: E-GEOD-13743 | ArrayExpress | 2014-05-01

SECONDARY ACCESSION(S): GSE13743PRJNA109393

REPOSITORIES: GEO, ArrayExpress

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Publications

Identification of stem cell transcriptional programs normally expressed in embryonic and neural stem cells in alloreactive CD8+ T cells mediating graft-versus-host disease.

Kato Koji K   Cui Shuaiying S   Kuick Rork R   Mineishi Shin S   Hexner Elizabeth E   Ferrara James L M JL   Emerson Stephen G SG   Zhang Yi Y  

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 20100129 6


A hallmark of graft-versus-host-disease (GVHD), a life-threatening complication after allogeneic hematopoietic stem cell transplantation, is the cytopathic injury of host tissues mediated by persistent alloreactive effector T cells (T(E)). However, the mechanisms that regulate the persistence of alloreactive T(E) during GVHD remain largely unknown. Using mouse GVHD models, we demonstrate that alloreactive CD8(+) T(E) rapidly diminished in vivo when adoptively transferred into irradiated secondar  ...[more]

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