Proteomics

Dataset Information

2

Bead-based kinase phosphorylation profiling identifies SRC as a therapeutic target in glioblastoma


ABSTRACT: Here we describe a bead-based method capable of profiling tyrosine kinase phosphorylations in a multiplexed, high-throughput and low-cost manner. This approach allows for the discovery of tyrosine kinase-activating events, even when the DNA sequence is wild-type. In an effort to pilot the establishment of a tyrosine kinase activation catalog, we profiled tyrosine phosphorylation levels of 62 tyrosine kinases in 130 human cancer lines, and followed-up on the frequent SRC phosphorylation in glioblastoma. Keywords: quantitative measurements of tyrosine phosphorylation levels on tyrosine kinases Total protein lysates were collected from 130 cancer cell lines. Tyrosine phosphorylation levels on 62 tyrosine kinases were measured with the bead assay.

ORGANISM(S): Homo sapiens  

SUBMITTER: Phioanh Nghiemphu   Andrew L Kung  Todd R Golub  Steven A Carr  Massimo Loda  Linda M Liau  Jinyan Du  Haley Hieronymous  Paula Bernasconi  Ingo K Mellinghoff  Rameen Beroukhim  David N Louis  Karl R Clauser  Bina Julian  Rebecca L Maglathin  Stephen P Finn  DR Mani  Timothy A Lewis  Xiao P Peng  Melissa Burns 

PROVIDER: E-GEOD-13808 | ArrayExpress | 2010-06-24

SECONDARY ACCESSION(S): GSE13808PRJNA110577

REPOSITORIES: GEO, ArrayExpress

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Publications


The aberrant activation of tyrosine kinases represents an important oncogenic mechanism, and yet the majority of such events remain undiscovered. Here we describe a bead-based method for detecting phosphorylation of both wild-type and mutant tyrosine kinases in a multiplexed, high-throughput and low-cost manner. With the aim of establishing a tyrosine kinase-activation catalog, we used this method to profile 130 human cancer lines. Follow-up experiments on the finding that SRC is frequently phos  ...[more]

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