Transcriptomics

Dataset Information

70

Repressive and active histone methylation mark distinct promoters in human and mouse spermatozoa (Nimblegen)


ABSTRACT: In higher eukaryotes, histone methylation is involved in the maintenance of cellular identity during somatic development. During spermatogenesis, most nucleosomes are replaced by protamines. Therefore, it is unclear if histone modifications function in paternal transmission of epigenetic information. Here we show that active H3K4 di-methylation (H3K4me2) and repressive H3K27 tri-methylation (H3K27me3), two modifications important for Trithorax and Polycomb-mediated gene regulation, are present in chromatin of human spermatozoa and show methylation-specific distributions at regulatory regions. H3K4me2-marked promoters control gene functions in spermatogenesis and cellular homeostasis suggesting that this mark reflects germline transcription. In contrast, H3K27me3 marks promoters of key developmental regulators in sperm as in soma. Many H3K27me3-marked genes are never expressed in the male and female germline, and in early “totipotent” embryos, suggesting a function for Polycomb in repressing somatic determinants across generations. Targets of H3K4me2 and H3K27me3 are also modified in mouse spermatozoa, implicating an evolutionary conserved role for histone methylation in chromatin inheritance via the male germline. Chromatin immuno precipitation (ChIP) was performed on sperm samples obtained from 9 normospermic donors. DNA associated with H3K4me2 or H3K27me3 was precipitated using specific antibodies. Input and precipitated DNA were amplified and hybridized to a tiling microarray (NimbleGen Systems Inc.) representing 18029 promoter regions (2200bp upstream to 500bp downstream of transcription start sites) of all RefSeq annotated human genes. For each modification 3 independent ChIP experiments were performed of which one was hybridized in a dye swap configuration.

ORGANISM(S): Homo sapiens  

SUBMITTER: Michael B Stadler   Urszula Brykczynska  Tim C Roloff  Mizue Hisano  Liliana Ramos  Antoine H Peters  Antoine Peters  Dirk Schübeler  Edward J Oakeley 

PROVIDER: E-GEOD-13863 | ArrayExpress | 2010-05-16

SECONDARY ACCESSION(S): GSE13863PRJNA123771

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Repressive and active histone methylation mark distinct promoters in human and mouse spermatozoa.

Brykczynska Urszula U   Hisano Mizue M   Erkek Serap S   Ramos Liliana L   Oakeley Edward J EJ   Roloff Tim C TC   Beisel Christian C   Schübeler Dirk D   Stadler Michael B MB   Peters Antoine H F M AH  

Nature structural & molecular biology 20100516 6


In higher eukaryotes, histone methylation is involved in maintaining cellular identity during somatic development. As most nucleosomes are replaced by protamines during spermatogenesis, it is unclear whether histone modifications function in paternal transmission of epigenetic information. Here we show that two modifications important for Trithorax- and Polycomb-mediated gene regulation have methylation-specific distributions at regulatory regions in human spermatozoa. Histone H3 Lys4 dimethylat  ...[more]

Similar Datasets

2010-05-16 | GSE13863 | GEO
2010-05-16 | GSE19889 | GEO
2010-05-16 | E-GEOD-19889 | ArrayExpress
| GSE49316 | GEO
2015-11-03 | E-GEOD-55471 | ArrayExpress
| GSE42629 | GEO
2013-06-15 | E-GEOD-42629 | ArrayExpress
2011-01-13 | GSE26609 | GEO
| GSE89502 | GEO
2011-01-13 | E-GEOD-26609 | ArrayExpress