Transcriptomics

Dataset Information

3

SiRNA profiling of human steroid-deprived LNCaP cells were transfected with control or ART-27 siRNA to investigate the genome-wide impact of ART-27 loss on androgen-regulated transcription


ABSTRACT: The androgen receptor (AR) directs diverse biological processes through interaction with coregulators such as androgen receptor trapped clone-27 (ART-27). The impact of ART-27 on genome-wide transcription was examined. The studies indicate that loss of ART-27 enhances expression of many androgen-regulated genes, suggesting that ART-27 inhibits gene expression. Surprisingly, classes of genes that are upregulated upon ART-27 depletion include regulators of DNA damage checkpoint and cell cycle progression, suggesting that ART-27 functions to keep expression levels of these genes low. Experiment Overall Design: Steroid-deprived LNCaP cells were transfected with control or ART-27 siRNA and stimulated with ethanol vehicle or 10 nM R1881 for 18 hrs. 8 samples, 4 conditions, 2 replicates per condition.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Susan Logan 

PROVIDER: E-GEOD-14043 | ArrayExpress | 2009-06-08

SECONDARY ACCESSION(S): GSE14043PRJNA112401

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Genome-wide impact of androgen receptor trapped clone-27 loss on androgen-regulated transcription in prostate cancer cells.

Nwachukwu Jerome C JC   Mita Paolo P   Ruoff Rachel R   Ha Susan S   Wang Qianben Q   Huang S Joseph SJ   Taneja Samir S SS   Brown Myles M   Gerald William L WL   Garabedian Michael J MJ   Logan Susan K SK  

Cancer research 20090324 7


The androgen receptor (AR) directs diverse biological processes through interaction with coregulators such as AR trapped clone-27 (ART-27). Our results show that ART-27 is recruited to AR-binding sites by chromatin immunoprecipitation analysis. In addition, the effect of ART-27 on genome-wide transcription was examined. The studies indicate that loss of ART-27 enhances expression of many androgen-regulated genes, suggesting that ART-27 inhibits gene expression. Surprisingly, classes of genes tha  ...[more]

Similar Datasets

2009-05-28 | GSE14043 | GEO
2015-08-27 | E-GEOD-11428 | ExpressionAtlas
2009-08-07 | E-GEOD-11428 | ArrayExpress
2011-08-28 | E-GEOD-28403 | ArrayExpress
2010-05-17 | E-GEOD-14092 | ArrayExpress
2012-11-30 | E-GEOD-39452 | ArrayExpress
2009-10-20 | E-GEOD-18401 | ArrayExpress
2007-08-17 | E-TABM-233 | ArrayExpress
2016-05-22 | E-GEOD-80450 | ArrayExpress
2014-10-21 | E-GEOD-58309 | ArrayExpress