Transcriptomics

Dataset Information

10

RNA pol II ChIP on chip (RPCC)


ABSTRACT: Adaptation of the ChIP-on-chip protocol, to calculate genomic transcription rates in S. cerevisiae. Keywords: ChIP-chip There are 3 different experimental conditions: -Yeast cell growing exponentially in YPD. -Yeast cell stopped after 2 h of changing them to YPGal. -Yeast cell growing exponentially after 14.5 h of changing them to YPGal. We have used 3 different IP protocols: -total RNApol II using a Myc tagged RNApol (RPB1-Myc) -RNApol II CTD using the Ab 8WG16 (Covance) -RNApol II CTD Phosphorilated on Ser5 (David Bentley\'s lab) There are 3 independent biological replicates of each experiment.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Vicent Pelechano   Jose E Perez-Ortin  Jose E. Perez-Ortin 

PROVIDER: E-GEOD-14060 | ArrayExpress | 2010-06-24

SECONDARY ACCESSION(S): GSE14060PRJNA114527

REPOSITORIES: GEO, ArrayExpress

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Publications

Regulon-specific control of transcription elongation across the yeast genome.

Pelechano Vicent V   Jimeno-González Silvia S   Rodríguez-Gil Alfonso A   García-Martínez José J   Pérez-Ortín José E JE   Chávez Sebastián S  

PLoS genetics 20090821 8


Transcription elongation by RNA polymerase II was often considered an invariant non-regulated process. However, genome-wide studies have shown that transcriptional pausing during elongation is a frequent phenomenon in tightly-regulated metazoan genes. Using a combination of ChIP-on-chip and genomic run-on approaches, we found that the proportion of transcriptionally active RNA polymerase II (active versus total) present throughout the yeast genome is characteristic of some functional gene classe  ...[more]

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