Transcriptomics

Dataset Information

5

RPCC analysis of rap1-sil, tpk1 & tpk2.


ABSTRACT: RPCC (RNA pol II ChIP-on-chip) experiments with different mutants that affect to the accumulation of non active RNA pol II along the yeast genome. Keywords: ChIP-chip There are 4 different strains: rap1-sil (without the silencing domain), RAP1 (both from Graham I.R. et al 1999) and tpk1 & tpk2 mutants (Euroscarf). The IP was done using an Ab against the RNApol II CTD (8WG16 Covance).There are 3 independent biological replicates of Rap1 and rap1-sil experiments and 2 for the tpk1 & tpk2 experiments.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Vicent Pelechano   Jose E Perez-Ortin  Jose E. Perez-Ortin 

PROVIDER: E-GEOD-14077 | ArrayExpress | 2010-06-24

SECONDARY ACCESSION(S): GSE14077PRJNA114625

REPOSITORIES: GEO, ArrayExpress

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Publications

Regulon-specific control of transcription elongation across the yeast genome.

Pelechano Vicent V   Jimeno-González Silvia S   Rodríguez-Gil Alfonso A   García-Martínez José J   Pérez-Ortín José E JE   Chávez Sebastián S  

PLoS genetics 20090821 8


Transcription elongation by RNA polymerase II was often considered an invariant non-regulated process. However, genome-wide studies have shown that transcriptional pausing during elongation is a frequent phenomenon in tightly-regulated metazoan genes. Using a combination of ChIP-on-chip and genomic run-on approaches, we found that the proportion of transcriptionally active RNA polymerase II (active versus total) present throughout the yeast genome is characteristic of some functional gene classe  ...[more]

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