Transcriptomics

Dataset Information

3

MESSAGE 2 space experiment with Rhodospirillum rubrum S1H


ABSTRACT: R. rubrum S1H inoculated on solid agar rich media was sent to the ISS in October 2003 (MESSAGE-part 2 experiment). After 10 days flight, R. rubrum cultures returned back to Earth. These cultures were then subjected to both transcriptomic and proteomic analysis and compared with the corresponding ground control. Whole-genome oligonucleotide microarray and high throughput proteomics, which offer the possibility to survey respectively the global transcriptional and translational response of an organism, were used to test the effect of space flight. Moreover, in an effort to identify a specific stress response of R. rubrum to space flight, ground simulation of space ionizing radiation and space gravity were performed under identical culture setup and growth conditions encountered during the actual space journey. This study is unique in combining the results from an actual space experiment with the corresponding space ionizing radiation and modeled microgravity ground simulations, which lead to a more solid dissection of the different factors contribution acting in space flight conditions. Total RNA was extracted from R. rubrum S1H grown after 10 days in space flight or after 10 days in simulated ionizing radiation or simulated microgravity. Each microarray slide contained 3 technical repeats.

ORGANISM(S): Rhodospirillum rubrum  

SUBMITTER: Natalie Leys  

PROVIDER: E-GEOD-14239 | ArrayExpress | 2010-05-16

SECONDARY ACCESSION(S): GSE14239PRJNA114453

REPOSITORIES: GEO, ArrayExpress

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Publications

Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight.

Mastroleo Felice F   Van Houdt Rob R   Leroy Baptiste B   Benotmane M Abderrafi MA   Janssen Ann A   Mergeay Max M   Vanhavere Filip F   Hendrickx Larissa L   Wattiez Ruddy R   Leys Natalie N  

The ISME journal 20090702 12


In view of long-haul space exploration missions, the European Space Agency initiated the Micro-Ecological Life Support System Alternative (MELiSSA) project targeting the total recycling of organic waste produced by the astronauts into oxygen, water and food using a loop of bacterial and higher plant bioreactors. In that purpose, the alpha-proteobacterium, Rhodospirillum rubrum S1H, was sent twice to the International Space Station and was analyzed post-flight using a newly developed R. rubrum wh  ...[more]

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