Transcriptomics

Dataset Information

3

Overexpression of NtmybA2 in tobacco BY-2 cells


ABSTRACT: Promoters of many G2/M phase-specific genes in plants contain mitosis-specific activator (MSA) elements, which act as G2/M phase-specific enhancers and bind with R1R2R3-Myb transcription factors. A least three R1R2R3-Myb proteins are expressed in tobacco (Nicotiana tabacum). To examine the genome-wide effects of NtmybA2 overexpression, we constructed transgenic tobacco BY-2 cell lines overexpressing full-length NtmybA2 protein. In addition, we generated transgenic BY-2 cell lines that overexpress truncated hyperactive form of NtmybA2 (NtmybA2∆C) lacking its C-terminal region which negatively regulates its own activity for transcriptional activation. We used a custom-made 16K cDNA microarray for comparative transcriptome analysis of these transgenic tobacco BY-2 cell lines. As control sample, BY-2 cells transformed with empty vector pPZP-35S were used. The transgenic cell lines were analyzed during stationary phase (i.e. day 7-8 after subculture into fresh media). Keywords: Genetic modification 1. pPZP-35S vector control, 2. NtmybA2 overexpressor, 3. C-truncated NtmybA2 (NtmyA2∆C) overexpressor

ORGANISM(S): Nicotiana tabacum  

SUBMITTER: Marie-Claire Criqui   Ivan Gális   Hiroo Fukuda  Masaki Ito  Taku Demura  Pascal Genschik  Thomas Potuschak  Satoshi Araki  Ken Matsuoka  Kiichi Kato  Shiori Suzuki 

PROVIDER: E-GEOD-14251 | ArrayExpress | 2010-05-22

SECONDARY ACCESSION(S): GSE14251PRJNA111181

REPOSITORIES: GEO, ArrayExpress

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Publications

Preferential up-regulation of G2/M phase-specific genes by overexpression of the hyperactive form of NtmybA2 lacking its negative regulation domain in tobacco BY-2 cells.

Kato Kiichi K   Gális Ivan I   Suzuki Shiori S   Araki Satoshi S   Demura Taku T   Criqui Marie-Claire MC   Potuschak Thomas T   Genschik Pascal P   Fukuda Hiroo H   Matsuoka Ken K   Ito Masaki M  

Plant physiology 20090225 4


Many G2/M phase-specific genes in plants contain mitosis-specific activator (MSA) elements, which act as G2/M phase-specific enhancers and bind with R1R2R3-Myb transcription factors. Here, we examined the genome-wide effects of NtmybA2 overexpression, one of the R1R2R3-Myb transcription factors in tobacco (Nicotiana tabacum). We used a custom-made 16-K cDNA microarray for comparative transcriptome analysis of transgenic tobacco BY-2 cell lines that overexpress NtmybA2 or its truncated hyperactiv  ...[more]

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