Genomics

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Global Mapping of Histone H3 K4 and K27 Trimethylation: Lineage Fate Determination of Differentiating CD4+ T Cells


ABSTRACT: Multipotential naïve CD4+ T cells differentiate into distinct lineages including Th1, Th2, Th17, and inducible T regulatory (iTreg) cells. The remarkable diversity of CD4+ T cells begs the question whether the observed changes reflect terminal differentiation with heritable epigenetic modifications or plasticity in T cell responses. We generated genome-wide histone H3 lysine 4 (H3K4) and lysine 27 (H3K27) trimethylation maps in naïve, Th1, Th2, Th17, iTreg, and natural (n)Treg cells. We find that while modifications of signature cytokine genes (Ifng, Il4, and Il17) partially conform to the expectation of lineage commitment, critical transcription factors such as Tbx21 exhibit a broad spectrum of epigenetic states, consistent with our demonstration of T-bet and IFN-γ induction in nTreg cells. Our data suggest an epigenetic mechanism underlying the specificity and plasticity of effector and regulatory T cells and also provide a framework for understanding complexity of CD4+ T helper cell differentiation. genome-wide analysis of histone H3 K4 and K27 trimethylation in different sub-lineages of mouse CD4+ T cells. (12 samples in total)

ORGANISM(S): Mus musculus  

SUBMITTER: Zhengju Yao  Weiqun Peng   Chongzhi Zang   Hong-Wei Sun   William E Paul   Tae-Young Roh   Jinfang Zhu   Jane Hu-Li   Gang Wei   Kairong Cui   Keji Zhao   John J O'Shea   Dustin E Schones   Wendy T Watford   Lai Wei   Yuka Kanno    

PROVIDER: E-GEOD-14254 | ArrayExpress | 2009-01-21

SECONDARY ACCESSION(S): GSE14254SRP000706PRJNA111207

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Global mapping of H3K4me3 and H3K27me3 reveals specificity and plasticity in lineage fate determination of differentiating CD4+ T cells.

Wei Gang G   Wei Lai L   Zhu Jinfang J   Zang Chongzhi C   Hu-Li Jane J   Yao Zhengju Z   Cui Kairong K   Kanno Yuka Y   Roh Tae-Young TY   Watford Wendy T WT   Schones Dustin E DE   Peng Weiqun W   Sun Hong-Wei HW   Paul William E WE   O'Shea John J JJ   Zhao Keji K  

Immunity 20090101 1


Multipotential naive CD4(+) T cells differentiate into distinct lineages including T helper 1 (Th1), Th2, Th17, and inducible T regulatory (iTreg) cells. The remarkable diversity of CD4(+) T cells begs the question whether the observed changes reflect terminal differentiation with heritable epigenetic modifications or plasticity in T cell responses. We generated genome-wide histone H3 lysine 4 (H3K4) and lysine 27 (H3K27) trimethylation maps in naive, Th1, Th2, Th17, iTreg, and natural Treg (nTr  ...[more]

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