Transcriptomics

Dataset Information

3

Transcription profiling of human quiescent keratinocytes and fibroblasts: radiation dose response


ABSTRACT: Skin is usually exposed during human exposures to ionizing radiation, however there are few experiments that evaluate the radiation responsiveness of the cells of the epidermis (keratinocytes) and those of the dermis (fibroblasts) in the same studies. We evaluated the transcriptional responses of quiesent primary keratinocytes and fibroblasts from the same individual and compared them with quiescent keratinocytes and fibroblasts that were immortalized by human telomerase (hTert). The primary transcriptional responses to 10-500 cGy ionizing radiation were p53-mediated responses; however, we did identify distinct responses between the keratinocytes and the fibroblasts. Experiment Overall Design: Four cell types (primary keratinocytes, hTert immortalized keratinocytes, primary fibroblasts, hTert immortalized fibroblasts) grown to quiescence, treated with 0, 10, 100 or 500 cGy gamma irradiation, RNA collected at 4 hrs.

ORGANISM(S): Homo sapiens  

SUBMITTER: Philip Moos  

PROVIDER: E-GEOD-14466 | ArrayExpress | 2009-06-08

SECONDARY ACCESSION(S): GSE14466PRJNA111465

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2009-05-31 | GSE14466 | GEO
2012-02-01 | E-GEOD-29344 | ArrayExpress
2014-10-21 | E-GEOD-58613 | ArrayExpress
2013-11-15 | E-GEOD-52403 | ArrayExpress
2013-11-30 | E-GEOD-48392 | ArrayExpress
2014-07-30 | E-GEOD-59861 | ArrayExpress
2014-07-30 | E-GEOD-59862 | ArrayExpress
| GSE56704 | GEO
2014-10-10 | E-GEOD-56704 | ArrayExpress
2013-08-16 | E-GEOD-49922 | ArrayExpress