Transcriptomics

Dataset Information

7

Gene profiling of Müller glia during early stages of zebrafish photoreceptor regeneration


ABSTRACT: Photoreceptor damage in adult mammals results in permanent cell loss and glial scarring in the retina. In contrast, adult zebrafish can regenerate photoreceptors following injury. By using a stable transgenic line in which GFP is driven by the cis-regulatory sequences of a glial specific marker gfap, Tg(gfap:GFP)mi2002, previous studies showed that Müller glia, the radial glial cells in the retina, proliferate after photoreceptor loss and give rise to neuronal progenitors that eventually differentiate into regenerated photoreceptors. To identify the molecular mechanisms that initiate this regenerative response, Müller glia were isolated from Tg(gfap:GFP)mi2002 fish during the early stages of regeneration after light lesion and gene expression profiles were generated by microarray analyses. Keywords: time course Retinas were dissected from Tg(gfap:GFP)mi2002 zebrafish at 8, 16, 24, 36 hour post-lesion (hpl) and non-light-treated controls (0 hpl) and were dissociated by enzymatic digestion. GFP+ Müller glia were isolated by fluorescence-activated cell sorting (FACS) for RNA extraction and hybridization on Affymetrix microarrays. Independent hybridization of three biological replicates were performed for each time point.

ORGANISM(S): Danio rerio  

SUBMITTER: Zhao Qin   Ritu Khanna  Pamela A Raymond  Matthew Brooks 

PROVIDER: E-GEOD-14495 | ArrayExpress | 2009-02-04

SECONDARY ACCESSION(S): GSE14495PRJNA111551

REPOSITORIES: GEO, ArrayExpress

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Publications

Genetic evidence for shared mechanisms of epimorphic regeneration in zebrafish.

Qin Zhao Z   Barthel Linda K LK   Raymond Pamela A PA  

Proceedings of the National Academy of Sciences of the United States of America 20090527 23


In a microarray-based gene profiling analysis of Müller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish, we found that 2 genes required for regeneration of fin and heart tissues in zebrafish, hspd1 (heat shock 60-kDa protein 1) and mps1 (monopolar spindle 1), were up-regulated. Expression of both genes in the neurogenic Müller glia and progenitors was independently verified by quantitative reverse transcriptase PCR and in situ hybridization. Functional analysis of t  ...[more]

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