Transcriptomics

Dataset Information

0

Transcription profiling of human HEK 293 cells after storage at ambient temperature and rehydration


ABSTRACT: This series represents the rehydration series of the human 293h media depleted storage on agarose / rehydration condition course analysis. Samples include Control Monolayer, 0 hr desiccation, 0 hr rehydration, 6 hr rehydration, 24 hr rehydration, and 72 hr rehydration.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Richard F. Helm  

PROVIDER: E-GEOD-1455 | ArrayExpress | 2008-06-11

SECONDARY ACCESSION(S): GSE1455PRJNA90005

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Long term metabolic arrest and recovery of HEK293 spheroids involves NF-kappaB signaling and sustained JNK activation.

Jack Graham D GD   Garst James F JF   Cabrera M Carla MC   DeSantis Andrea M AM   Slaughter Stephen M SM   Jervis Jody J   Brooks Andrew I AI   Potts Malcolm M   Helm Richard F RF  

Journal of cellular physiology 20060201 2


Understanding how cells withstand a depletion of intracellular water is relevant to the study of longevity, aging, and quiescence because one consequence of air-drying is metabolic arrest. After removal of medium, HEK293 spheroids with intracellular water content of approximately 65% survived partial vacuum, with antistatic control, for weeks in the dark at 25 degrees C. In contrast, only a limited exposure of monolayers to air was lethal; the mitochondrion being a target of this stress. The pat  ...[more]

Similar Datasets

2005-08-01 | GSE1455 | GEO
2008-06-11 | E-GEOD-1364 | ArrayExpress
2007-10-23 | E-GEOD-1313 | ArrayExpress
2007-10-23 | E-GEOD-1312 | ArrayExpress
2007-10-23 | E-GEOD-1311 | ArrayExpress
2016-06-19 | MSV000079840 | Massive
2013-11-25 | E-GEOD-47624 | ArrayExpress
| GSE4217 | GEO
| GSE4218 | GEO
2014-04-15 | E-MTAB-914 | ArrayExpress