Transcriptomics,Genomics

Dataset Information

297

Extensive demethylation of repetitive elements during seed development underlies gene imprinting


ABSTRACT: DNA methylation is an epigenetic mark associated with transposable element silencing and gene imprinting in flowering plants and mammals. In plants, imprinting occurs in the endosperm, which nourishes the embryo during seed development. We have profiled Arabidopsis DNA methylation genome-wide in the embryo and endosperm. Large-scale methylation changes accompany endosperm development and endosperm-specific gene expression. Transposable element fragments are extensively demethylated in the endosperm. We discovered new imprinted genes by identifying candidate genes associated with the top differentially methylated regions. Our data suggest that imprinting in plants evolved from genome defense against transposable elements. Keywords: Affinity-purification on microarray All experiments were done using two channels per chip. Immunoprecipitated methylated DNA (IP) was compared to control genomic DNA (input). Both the IP and input represent Cy5 and Cy3 labeled Illumina GA libraries.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Steve Henikoff   Jorja Henikoff  Mary Gehring 

PROVIDER: E-GEOD-14570 | ArrayExpress | 2009-06-12

SECONDARY ACCESSION(S): GSE14570SRP000779PRJNA111577

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Extensive demethylation of repetitive elements during seed development underlies gene imprinting.

Gehring Mary M   Bubb Kerry L KL   Henikoff Steven S  

Science (New York, N.Y.) 20090601 5933


DNA methylation is an epigenetic mark associated with transposable element silencing and gene imprinting in flowering plants and mammals. In plants, imprinting occurs in the endosperm, which nourishes the embryo during seed development. We have profiled Arabidopsis DNA methylation genome-wide in the embryo and endosperm and found that large-scale methylation changes accompany endosperm development and endosperm-specific gene expression. Transposable element fragments are extensively demethylated  ...[more]

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