Transcriptomics

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Understanding adult human progenitor cells


ABSTRACT: Overarching aim is to achieve a greater understanding of the control of progenitor cells within the adult human retina within the normal and diseased retinal microenvironment. Specifically we will assess via our experimental designs: (i) the control of CD133+ retinal cell populations that display mitotic potential and differentiation and Keywords: comparative genomic hybridization using adult human CD133+ & CD133- retinal cells Adult human retina was processed to produce a cell suspension, CD133+ cells were magnetically isolated to produce cell populations of CD133+ and CD133- cells. 3 retinal samples were analysed for each cell population.

ORGANISM(S): Homo sapiens  

SUBMITTER: Debra A Carter   DEBRA ANN CARTER  Eric J Mayer  Andrew D Dick 

PROVIDER: E-GEOD-14733 | ArrayExpress | 2009-02-06

SECONDARY ACCESSION(S): GSE14733PRJNA112193

REPOSITORIES: GEO, ArrayExpress

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Publications

CD133+ adult human retinal cells remain undifferentiated in Leukaemia Inhibitory Factor (LIF).

Carter Debra A DA   Dick Andrew D AD   Mayer Eric J EJ  

BMC ophthalmology 20090223


CD133 is a cell surface marker of haematopoietic stem and progenitor cells. Leukaemia inhibitory factor (LIF), sustains proliferation and not differentiation of embryonic stem cells. We used CD133 to purify adult human retinal cells and aimed to determine what effect LIF had on these cultures and whether they still had the ability to generate neurospheres.Retinal cell suspensions were derived from adult human post-mortem tissue with ethical approval. With magnetic automated cell sorting (MACS) C  ...[more]

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