Transcriptomics

Dataset Information

30

H. pylori G27 HP0518 mutant showed greater motility


ABSTRACT: Helicobacter pylori is a human gastric pathogen associated with gastric and duodenal ulcers as well as specific gastric cancers. It is well-evidenced that motility is essential for this pathogen to colonize human gastric tissues. We found that the H. pylori G27 HP0518 mutant showed greater motility than the parental strain, leading to increased cell adhesion and subsequent CagA translocation and NF-κB activation in AGS cells. This mutant expressed a higher molecular mass flagellin A (FlaA) than the parental wild-type strain and the complemented HP0518 mutant, which correlated with differences in motility. Deglycosylation assays indicated that the increased molecular mass of the FlaA protein expressed by the mutant was due to O-linked glycoside modifications. Electron micrographs demonstrated that expression of bundle-formed flagellin filaments in the HP0518 mutant was enhanced in comparison to the wild-type strain. Different degrees of FlaA glycosylation between H. pylori strains suggested that glycosylation could affect both virulence and persistence in vivo. In conclusion, HP0518 inactivation resulted in FlaA hyper-glycosylation leading to increased virulence and motility. Microarray experiments were carried out as two-color hybridizations with a color-swap dye-reversal setting to compensate Cy-dye specific effects and to ensure statistically relevant data.

ORGANISM(S): Helicobacter pylori  

SUBMITTER: Hans-Joachim Mollenkopf  Hans Molenkopf   Thomas F Meyer   Hiroshi Asakura    

PROVIDER: E-GEOD-14752 | ArrayExpress| 2014-05-01

SECONDARY ACCESSION(S): GSE14752PRJNA112243

REPOSITORIES: GEO, ArrayExpress

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Publications

Helicobacter pylori HP0518 affects flagellin glycosylation to alter bacterial motility.

Asakura Hiroshi H   Churin Yuri Y   Bauer Bianca B   Boettcher Jan Peter JP   Bartfeld Sina S   Hashii Noritaka N   Kawasaki Nana N   Mollenkopf Hans J HJ   Jungblut Peter R PR   Brinkmann Volker V   Meyer Thomas F TF  

Molecular microbiology 20100930 5


Helicobacter pylori is a human gastric pathogen associated with gastric and duodenal ulcers as well as gastric cancer. Mounting evidence suggests this pathogen's motility is prerequisite for successful colonization of human gastric tissues. Here, we isolated an H. pylori G27 HP0518 mutant exhibiting altered motility in comparison to its parental strain. We show that the mutant's modulated motility is linked to increased levels of O-linked glycosylation on flagellin A (FlaA) protein. Recombinant  ...[more]

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