Transcriptomics

Dataset Information

5

Transcription profiling by array of Arabidopsis mutant for gapcp


ABSTRACT: Glycolytic Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde 3-phospate to 1,3-bisphosphoglycerate by coupling with the reduction of NAD+ to NADH. Both cytosolic and plastidial isoforms of GAPDH has been described but the in vivo functions of the plastidial isoforms is unresolved. We generated mutants of the Arabidopsis plastidial GAPDH isoforms (At1g79530, At1g16300; GAPCp1, GAPCp2) and performed a microarray analysis comparing gapcp double (gapcp1 gapcp2) mutant and wild type seedlings Experiment Overall Design: 15-day old Arabidopsis seedlings (Col 0) and gapcp1gapcp2 double mutants were used for RNA extraction and hybridization on Affymetrix microarrays.

REANALYSED by: E-GEOD-14765

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Roc Ros  

PROVIDER: E-GEOD-14765 | ArrayExpress | 2009-02-21

SECONDARY ACCESSION(S): GSE14765PRJNA112263

REPOSITORIES: GEO, ArrayExpress

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