Dataset Information


Global gene expression of the colonic epithelium from proximal and distal sections of azoxymethane and saline-treated Sprague Dawley rats

ABSTRACT: The rat models of colorectal cancer (CRC), such as the azoxymethane (AOM) cancer-inducing model, are important tools for researching cancer initiation pathways. However, there is limited understanding of the expression pathways of underlying normal rat colonic epithelium and how this relates to human colonic epithelium. The aim of this study was to study the acute effects of AOM on the gene and pathway expression of the rat's colonic epithelium, whilst contrasting the background normal global expression patterns along the length of the rat as compared to the normal human colonic epithelium. The study used microarrays to investigate global gene expression of the colonic epithelium from proximal and distal sections of AOM- and saline (normal)-treated Sprague Dawley rats. Rat gene and pathway expression patterns were then compared in-silico with human microarray data (see GSE9254 for files) from normal tissue samples originating from proximal and distal regions of the colon.


ORGANISM(S): Rattus norvegicus  

SUBMITTER: Richard Head   Trevor Lockett  William J Wilson  Lauren M Bragg  Julie Clarke  Rob Dunne  Barney M Hines  Caroline A Kerr  Janet Shaw  Caroline Audrey Kerr 

PROVIDER: E-GEOD-15184 | ArrayExpress | 2010-06-16



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Genomic homeostasis is dysregulated in favour of apoptosis in the colonic epithelium of the azoxymethane treated rat.

Kerr Caroline A CA   Hines Barney M BM   Shaw Janet M JM   Dunne Robert R   Bragg Lauren M LM   Clarke Julie J   Lockett Trevor T   Head Richard R  

BMC physiology 20130123

BACKGROUND: The acute response to genotoxic carcinogens in rats is an important model for researching cancer initiation events. In this report we define the normal rat colonic epithelium by describing transcriptional events along the anterior-posterior axis and then investigate the acute effects of azoxymethane (AOM) on gene expression, with a particular emphasis on pathways associated with the maintenance of genomic integrity in the proximal and distal compartments using whole genome expression  ...[more]

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