Dataset Information


Gene expression profile of ARPE-19 cells treated with TGF and TNF for 6 and 42 hour.

ABSTRACT: Aberrant epithelial-mesenchymal transition (EMT) is involved in pathological processes including fibrotic disorders and cancer invasion and metastasis. Alterations of the cell-extracellular matrix (ECM) interaction also contribute to those pathological settings. However, the functional interplay between EMT and cell-ECM interaction is poorly understood. Here, we show that tumor necrosis factor (TNF)-alpha, a potent mediator of inflammation, induces EMT-associated fibrosis in retinal pigment epithelial cells, and that this is regulated by hyaluronan (HA)-CD44-Moesin interaction. TNF-alpha elicits both HA synthesis and Moesin phosphorylation through protein kinase C activation, promoting binding of CD44 to the newly synthesized HA. The HA-CD44-Moesin interaction leads to cell-cell dissociation through actin remodeling and increased cellular motility associated with mesenchymal phenotype. Furthermore, we established an in vivo model of TNF-alpha-induced fibrosis in the mouse eye, and the ocular fibrosis was completely suppressed in CD44-null mice. Therefore, HA production and its interaction with CD44 plays essential role in TNF-alpha-induced-EMT, and the interference of the complex formation can be a new strategy for the fibrotic disorders. ARPE19 cell lines were treated with TGF and TNF for 6 and 42 hour. Each experiment were repeated three times. But 1hour experiment was repeated two times. For this submission, total RNA was extracted from TGF- or TNF-treated ARPE-19 cells and differential gene expression between each time point (6 and 42 hours) was determined using genechip arrays (Affymetrix, Human Genome U133).


ORGANISM(S): Homo sapiens  


PROVIDER: E-GEOD-15205 | ArrayExpress | 2010-03-23



Similar Datasets

2010-03-11 | GSE15205 | GEO
2015-09-02 | E-GEOD-15205 | ExpressionAtlas
2013-11-01 | E-GEOD-51938 | ArrayExpress
2008-06-12 | E-GEOD-2705 | ArrayExpress
| GSE89633 | GEO
2012-10-31 | E-GEOD-33416 | ArrayExpress
2018-03-26 | PXD006179 | Pride
| GSE61220 | GEO
2015-02-25 | E-GEOD-66278 | ArrayExpress
2010-06-01 | GSE21064 | GEO