Transcriptomics,Multiomics

Dataset Information

3

High-throughput sequencing of small RNAs in chicken somite embryos


ABSTRACT: High-throughput sequencing of small RNAs in chicken somite embryos Dissected tissue white leghorn chicken embryos was disaggregated and RNA extracted using the miRVana kit (Ambion). Small RNA fraction between 19 and 24 nt was isolated from 15% denaturing polyacrylamide gel and 15 micro gram was ligated to Solexa adaptors (Illumina) without de-phosphorylating and re-phosphorylating. The short RNAs were converted to DNA by RT-PCR following the Illumina protocol.

OTHER RELATED OMICS DATASETS IN: PRJNA115677

ORGANISM(S): Gallus Gallus

SUBMITTER: Helio Pais   Tamas Dalmay  Dylan Sweetman  Vincent Moulton  Tina Rathjen  Andrea Munsterberg 

PROVIDER: E-GEOD-15513 | ArrayExpress | 2009-04-27

SECONDARY ACCESSION(S): GSE15513SRP001271PRJNA115677

REPOSITORIES: GEO, ArrayExpress, ENA

Dataset's files

Source:
Action DRS
E-GEOD-15513.README.txt Txt
E-GEOD-15513.additional.1.zip Other
E-GEOD-15513.idf.txt Idf
E-GEOD-15513.processed.1.zip Processed
E-GEOD-15513.sdrf.txt Txt
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Publications

High throughput sequencing of microRNAs in chicken somites.

Rathjen Tina T   Pais Helio H   Sweetman Dylan D   Moulton Vincent V   Munsterberg Andrea A   Dalmay Tamas T  

FEBS letters 20090327 9


High throughput Solexa sequencing technology was applied to identify microRNAs in somites of developing chicken embryos. We obtained 651,273 reads, from which 340,415 were mapped to the chicken genome representing 1701 distinct sequences. Eighty-five of these were known microRNAs and 42 novel miRNA candidates were identified. Accumulation of 18 of 42 sequences was confirmed by Northern blot analysis. Ten of the 18 sequences are new variants of known miRNAs and eight short RNAs are novel miRNAs.  ...[more]

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