Genomics

Dataset Information

296

Identification of transcription factor MAB-5 binding sites


ABSTRACT: We identified the DNA binding sites of MAB-5 in C. elegans. MAB-5 was C-terminally tagged with GFP and the expression pattern was examined through fluorescent microscopy. The binding sites were determined using chromatin immunoprecipitation with anti-GFP antibody followed by illumina high-throughput sequencing (ChIP-seq). For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Identification of MAB-5 binding sites at L3 stage

ORGANISM(S): Caenorhabditis elegans  

SUBMITTER: Mei Zhong   Michael P Wilson  Wei Niu 

PROVIDER: E-GEOD-15625 | ArrayExpress | 2009-04-11

SECONDARY ACCESSION(S): GSE15625SRP000865

REPOSITORIES: GEO, ArrayExpress, ENA

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Regulation of gene expression by sequence-specific transcription factors is central to developmental programs and depends on the binding of transcription factors with target sites in the genome. To date, most such analyses in Caenorhabditis elegans have focused on the interactions between a single transcription factor with one or a few select target genes. As part of the modENCODE Consortium, we have used chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-seq) to det  ...[more]

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