Transcriptomics,Multiomics

Dataset Information

3

Chromosomal duplication in the grandifolia-D mutants of Arabidopsis thaliana, expression data


ABSTRACT: The number of cells in an organ is a major factor for the determination of organ size. However, genetic basis of cell number determination is not well understood. Three grandifolia-D (gra-D) mutants of Arabidopsis thaliana developed huge leaves containing two- to three-fold increased number of cells of the wild type. Tiling array and microarray analysis of gra-D mutants suggested that genes found in a lower part of chromosome 4 were upregulated, suggesting the occurrence of segmental chromosomal duplications in the gra-D mutants. These region contain positive regulators of cell proliferation such as AINTEGUMENTA (ANT) and cyclin genes such as CYCD3;1. Total RNAs were extracted using RNeasy plant mini kit (Qiagen) from vegetative part of seedlings at stage 1.03 grown in vitro. RNAs from three biological repeats of wild type (wt, control) and the 3 grandifolia mutants (gra1-D, gra2-D and gra3-D) were submitted to ATH1 array hybridization.

OTHER RELATED OMICS DATASETS IN: PRJNA122997PRJNA116617GSE15726PRJNA122999

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Gerrit T Beemster   Gorou Horiguchi  Hirokazu Tsukaya  Dirk Inzé  Nathalie Gonzalez 

PROVIDER: E-GEOD-15722 | ArrayExpress | 2010-05-18

SECONDARY ACCESSION(S): GSE15722PRJNA122997

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-15722.README.txt Txt
E-GEOD-15722.eSet.r Other
E-GEOD-15722.idf.txt Idf
E-GEOD-15722.processed.1.zip Processed
E-GEOD-15722.raw.1.zip Raw
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Publications

Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana.

Horiguchi Gorou G   Gonzalez Nathalie N   Beemster Gerrit T S GT   Inzé Dirk D   Tsukaya Hirokazu H  

The Plant journal : for cell and molecular biology 20090605 1


The number of cells in an organ is a major factor that specifies its size. However, the genetic basis of cell number determination is not well understood. To obtain insight into this genetic basis, three grandifolia-D (gra-D) mutants of Arabidopsis thaliana were characterized that developed huge leaves with two to three times more cells than the wild-type. Genetic and microarray analyses showed that a large segmental duplication had occurred in all the gra-D mutants, consisting of the lower part  ...[more]

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