Dataset Information


Halobacterium salinarum NRC-1 total RNA hybridization of TFBd overexpression versus Reference sample

ABSTRACT: Total RNA hybridizations of Halobacterium salinarum NRC-1 total RNA versus a strain overexpressing TFBd were compared to verify the transcriptome landscape changes. Comparison with TFBd binding sites localized using ChIP-chip data and MeDiChI algorithm (Reiss et al, 2008) allowed observation that TF-binding in the middle of coding sequences can also result in transcription initiation or termination internal to a single annotated protein-coding gene. Total RNA sample of plasmid based expression of Cmyc tagged transcription factor TFBd grown in standard growth media with mevinolin.

ORGANISM(S): Halobacterium salinarum NRC-1  

SUBMITTER: Tie Koide  Min Pan  David J Reiss  Nitin S Baliga  Marc T Facciotti 

PROVIDER: E-GEOD-15788 | ArrayExpress | 2010-05-17



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Despite the knowledge of complex prokaryotic-transcription mechanisms, generalized rules, such as the simplified organization of genes into operons with well-defined promoters and terminators, have had a significant role in systems analysis of regulatory logic in both bacteria and archaea. Here, we have investigated the prevalence of alternate regulatory mechanisms through genome-wide characterization of transcript structures of approximately 64% of all genes, including putative non-coding RNAs  ...[more]

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