Transcriptomics

Dataset Information

5

Halobacterium salinarum NRC-1 total RNA hybridization of TFBd overexpression versus Reference sample


ABSTRACT: Total RNA hybridizations of Halobacterium salinarum NRC-1 total RNA versus a strain overexpressing TFBd were compared to verify the transcriptome landscape changes. Comparison with TFBd binding sites localized using ChIP-chip data and MeDiChI algorithm (Reiss et al, 2008) allowed observation that TF-binding in the middle of coding sequences can also result in transcription initiation or termination internal to a single annotated protein-coding gene. Total RNA sample of plasmid based expression of Cmyc tagged transcription factor TFBd grown in standard growth media with mevinolin.

ORGANISM(S): Halobacterium salinarum NRC-1  

SUBMITTER: Tie Koide  Min Pan  David J Reiss  Nitin S Baliga  Marc T Facciotti 

PROVIDER: E-GEOD-15788 | ArrayExpress | 2010-05-17

SECONDARY ACCESSION(S): GSE15788PRJNA116867

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications


Despite the knowledge of complex prokaryotic-transcription mechanisms, generalized rules, such as the simplified organization of genes into operons with well-defined promoters and terminators, have had a significant role in systems analysis of regulatory logic in both bacteria and archaea. Here, we have investigated the prevalence of alternate regulatory mechanisms through genome-wide characterization of transcript structures of approximately 64% of all genes, including putative non-coding RNAs  ...[more]

Similar Datasets

2010-05-17 | E-GEOD-12977 | ArrayExpress
2010-02-10 | E-GEOD-14976 | ArrayExpress
2010-06-19 | E-GEOD-15272 | ArrayExpress
2010-10-08 | E-GEOD-15275 | ArrayExpress
2010-06-19 | E-GEOD-15273 | ArrayExpress
2010-06-19 | E-GEOD-15274 | ArrayExpress
2010-05-26 | E-GEOD-17515 | ArrayExpress
2009-04-14 | E-GEOD-15282 | ArrayExpress
2010-06-19 | E-GEOD-15276 | ArrayExpress
2010-05-17 | E-GEOD-13150 | ArrayExpress