Dataset Information


Transcription profiling by array of Drosophila ovary somatic sheet cells

ABSTRACT: Piwi-interacting RNAs (piRNAs) are ~24-30 nucleotide regulatory RNAs that are abundantly expressed in gonads. The most well-understood piRNAs mediate post-transcriptional defense against transposable elements (TEs), and derive from sense or antisense strands as a consequence of "ping-pong" amplification of complementary sequences of active TEs and piRNA master control transcripts. Another class of piRNAs, such as those expressed in pachytene testis, derive from large intergenic clusters that are strictly single-stranded. Here, we report a third substrate that generates abundant primary piRNAs. In somatic follicle cells of Drosophila ovaries, we cloned >1 million piRNAs from thousands of messenger RNAs, and these were quite preferentially derived from 3' untranslated regions. This segregation implies a competition between the translation machinery and primary piRNA biogenesis machinery for mRNA access. 3 replicates.

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Nicolas Robine   Eric C Lai  Sudha Balla  Nelson Lau 

PROVIDER: E-GEOD-15825 | ArrayExpress | 2010-01-03



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A broadly conserved pathway generates 3'UTR-directed primary piRNAs.

Robine Nicolas N   Lau Nelson C NC   Balla Sudha S   Jin Zhigang Z   Okamura Katsutomo K   Kuramochi-Miyagawa Satomi S   Blower Michael D MD   Lai Eric C EC  

Current biology : CB 20091201 24

BACKGROUND:Piwi-interacting RNAs (piRNAs) are approximately 24-30 nucleotide regulatory RNAs that are abundant in animal gonads and early embryos. The best-characterized piRNAs mediate a conserved pathway that restricts transposable elements, and these frequently engage a "ping-pong" amplification loop. Certain stages of mammalian testis also accumulate abundant piRNAs of unknown function, which derive from noncoding RNAs that are depleted in transposable element content and do not engage in pin  ...[more]

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