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Analysis of human myeloma cell lines expressing beta 3 integrin

ABSTRACT: The aim of these experiments was designed to compare gene expression in human myeloma cell lines expressing beta 3 integrin vs counterpart cell lines that do not express beta 3 integrin. Keywords: Gene expression,cell lines, siRNA Phenotype characterization of both primary and cultured myeloma plasma cells was assessed by flow cytometry (FACScanto, Becton Dickinson, San Jose, CA) using a panel of MoAbs or antisera to the following markers: CD138, CD38, CD56, k/l chains, CD20, CD44, CD54, alphav and beta3 chains. The microarray analysis was performed on: myeloma bone resorbing cell lines alphav-beta3 positive and the same cell line silenced for the integrins; myeloma non bone resorbing cell lines negative for the expression of avb3.

ORGANISM(S): Homo sapiens  

SUBMITTER: Marco Tucci   gabriella rodolico  Gabriella Rodolico  Raffaele De Palma  Francesco Silvestris 

PROVIDER: E-GEOD-15867 | ArrayExpress | 2010-05-19



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beta(3) Integrin subunit mediates the bone-resorbing function exerted by cultured myeloma plasma cells.

Tucci Marco M   De Palma Raffaele R   Lombardi Lucia L   Rodolico Gabriella G   Berrino Liberato L   Dammacco Franco F   Silvestris Franco F  

Cancer research 20090804 16

alpha(v)beta(3) integrin was investigated in multiple myeloma in relation to the in vitro osteoclast-like activity of malignant plasma cells. Myeloma cells from patients with skeleton involvement overexpressed alpha(v)beta(3) and produced erosion pits on bone substrates, whereas this effect was not observed by cells from patients with no evidence of bone disease. We therefore explored the alpha(v)beta(3) transcriptional pathway in the bone-resorbing cells. Silencing of beta(3) chain abrogated th  ...[more]

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