Dataset Information

Down-regulation of SFRP1 in a mammary epithelial cell line

ABSTRACT: Microarray data to accompany Gauger et al. (2009) Cancer Cell International paper A treated X untreated design was used whereby immortalized human mammary epithelial cell lines (TERT cells) were modified to stably express an siRNA that knocked down SFRP1 expression. These "treated" cells were labeled with Cy5 and four replicate harvests were hybridized to Agilent 4by44k whole genome microarrays, along with a Cy3 labeled untreated control. The untreated control consisted of 4 pooled harvests of the parent cell line in normal growth conditions at 60-80% confluence.

ORGANISM(S): Homo sapiens

SUBMITTER: Melissa Troester

PROVIDER: E-GEOD-15948 | ArrayExpress | 2010-05-08

SECONDARY ACCESSION(S): GSE15948PRJNA117005

REPOSITORIES: GEO, ArrayExpress

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Publications

Down-regulation of sfrp1 in a mammary epithelial cell line promotes the development of a cd44high/cd24low population which is invasive and resistant to anoikis.

Gauger Kelly J KJ Hugh Jeremy M JM Troester Melissa A MA Schneider Sallie Smith SS

Cancer Cell International 20090507

BACKGROUND: The Wnt family of secreted proteins is implicated in the regulation of cell fate during development, as well as in cell proliferation, morphology, and migration. Aberrant activation of the Wnt/beta-catenin signaling pathway leads to the development of several human cancers, including breast cancer. Secreted frizzled-related protein 1 (SFRP1) antagonizes this pathway by competing with the Frizzled receptor for Wnt ligands resulting in an attenuation of the signal transduction cascade. ...[more]

PMID: 19422694

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