Dataset Information


Transcription profiling of human bladder smooth muscle cells subjected to cyclic stretch-relaxation

ABSTRACT: The aim of the experiment was to determine the effect of cyclic stretch-relaxation ("stretch") on gene expression patterns in normal diploid human bladder smooth muscle cells. Cells plated on silicone elastomer bottomed 6-well culture dishes were grown to ~80% confluence, serum-depleted for 48h and subjected to cyclic stretch-relaxation at 20% elongation for 4h. Cells seeded in stretch plates but not subjected to stretch served as controls. Total RNA was extracted from both groups of cells, reverse-transcribed, biotin-labeled, fragmented and hybridized to HG-U133A. Four biological replicates were generated for each treatment group (non-stretched or stretched).

ORGANISM(S): Homo sapiens  

SUBMITTER: Rosalyn Adam  

PROVIDER: E-GEOD-1595 | ArrayExpress | 2007-08-04



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Mechanical stretch is a highly selective regulator of gene expression in human bladder smooth muscle cells.

Adam Rosalyn M RM   Eaton Samuel H SH   Estrada Carlos C   Nimgaonkar Ashish A   Shih Shu-Ching SC   Smith Lois E H LE   Kohane Isaac S IS   Bägli Darius D   Freeman Michael R MR  

Physiological genomics 20041005 1

Application of mechanical stimuli has been shown to alter gene expression in bladder smooth muscle cells (SMC). To date, only a limited number of "stretch-responsive" genes in this cell type have been reported. We employed oligonucleotide arrays to identify stretch-sensitive genes in primary culture human bladder SMC subjected to repetitive mechanical stimulation for 4 h. Differential gene expression between stretched and nonstretched cells was assessed using Significance Analysis of Microarrays  ...[more]

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