Transcriptomics,Multiomics

Dataset Information

88

Gene expression profile in mouse embryonic stem cells in which endogenous Pcl2 expression was stably knocked down using short hairpin RNA


ABSTRACT: Polycomb group (PcG) proteins are highly conserved epigenetic transcriptional repressors important for the control of numerous developmental gene expression programs and have recently been implicated in the modulation of embryonic stem cell (ESC) identity. We identified the PcG protein PCL2 (polycomb-like 2) in a genome-wide screen for novel regulators of self-renewal and pluripotency and predicted that it would play an important role in mouse ESC fate determination. Using multiple biochemical strategies, we provide evidence that PCL2 is a novel Polycomb Repressive Complex 2 (PRC2)-associated protein in mouse ESCs. Knockdown of Pcl2 in ESCs resulted in heightened self-renewal characteristics, defects in differentiation and altered patterns of histone methylation. Through integration of global gene expression and promoter occupancy analyses of both PCL2 and PRC2 components EZH2 and SUZ12, we have predicted PCL2 target genes and formulated regulatory networks describing the role of PCL2 both in modulating transcription of ESC self-renewal genes in undifferentiated ESCs as well as developmental regulators during early commitment and differentiation. Cells were stably expressing Pcl2 shRNA or shRNA mismatch control sequences. Hybridizations of three biological replicates for both the control and Pcl2 shRNA clone were performed.

REANALYSED by: E-GEOD-16364

ORGANISM(S): Mus musculus  

SUBMITTER: Gerard Cagney  Joseph Torchia   Nevan Krogan   Kamal Garcha   Wing Y Chang   Julie Hunkapiller   Emily Walker   Jeremy Reiter   William L Stanford    

PROVIDER: E-GEOD-16364 | ArrayExpress| 2015-09-03

SECONDARY ACCESSION(S): GSE16364PRJNA115321

REPOSITORIES: GEO, ArrayExpress

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Publications

Polycomb-like 2 associates with PRC2 and regulates transcriptional networks during mouse embryonic stem cell self-renewal and differentiation.

Walker Emily E   Chang Wing Y WY   Hunkapiller Julie J   Cagney Gerard G   Garcha Kamal K   Torchia Joseph J   Krogan Nevan J NJ   Reiter Jeremy F JF   Stanford William L WL  

Cell stem cell 20100201 2


Polycomb group (PcG) proteins are conserved epigenetic transcriptional repressors that control numerous developmental gene expression programs and have recently been implicated in modulating embryonic stem cell (ESC) fate. We identified the PcG protein PCL2 (polycomb-like 2) in a genome-wide screen for regulators of self-renewal and pluripotency and predicted that it would play an important role in mouse ESC-fate determination. Using multiple biochemical strategies, we provide evidence that PCL2  ...[more]

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