Dataset Information


Transcription profiling by array of Arabidopsis mesophyll cells after treatment with flg22

ABSTRACT: The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis mesophyll cells that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression underlying intial cellular and molecular responses to microbial associated molecualr patterns (MAMPs). Arabidopsis mesophyll protoplasts were treated with 100 nM flg22 for 30 min and 60 min. Transcript profiles were analyzed and compared between control and treated cells to identify early flg22 responsive genes.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Jen Sheen   Matthew McCormack  Horim Lee 

PROVIDER: E-GEOD-16472 | ArrayExpress | 2010-03-18



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Differential innate immune signalling via Ca(2+) sensor protein kinases.

Boudsocq Marie M   Willmann Matthew R MR   McCormack Matthew M   Lee Horim H   Shan Libo L   He Ping P   Bush Jenifer J   Cheng Shu-Hua SH   Sheen Jen J  

Nature 20100217 7287

Innate immunity represents the first line of inducible defence against microbial infection in plants and animals. In both kingdoms, recognition of pathogen- or microbe-associated molecular patterns (PAMPs or MAMPs, respectively), such as flagellin, initiates convergent signalling pathways involving mitogen-activated protein kinase (MAPK) cascades and global transcriptional changes to boost immunity. Although Ca(2+) has long been recognized as an essential and conserved primary mediator in plant  ...[more]

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