Dataset Information


Tumor T17 Sectors

ABSTRACT: The following CGH experiments were conducted on four sectors (S1-S4) from a single primary ductal carcinoma tumor (T17) using the Sector-Ploidy-Profiling (SPP) Approach. SPP involves macro-dissecting the tumor, flow-sorting nuclei by differences in total genomic DNA content and profiling the genome of the tumor subpopulations. The genomic DNA from each tumor subpopulation was labeled with Cy5 and hybridized to an 390K Rubble-Rep ROMA Microarray. A normal reference male fibroblast was labeled with Cy3 as a control. Samples were cohybridize to the same single microarray.

SUBMITTER: Nicholas Navin 

PROVIDER: E-GEOD-16665 | ArrayExpress | 2010-05-16



Dataset's files

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E-GEOD-16665.README.txt Txt
E-GEOD-16665.idf.txt Idf Processed Raw
E-GEOD-16665.sdrf.txt Txt
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High-resolution nucleosome mapping reveals transcription-dependent promoter packaging.

Weiner Assaf A   Hughes Amanda A   Yassour Moran M   Rando Oliver J OJ   Friedman Nir N  

Genome research 20091021 1

Genome-wide mapping of nucleosomes has revealed a great deal about the relationships between chromatin structure and control of gene expression, and has led to mechanistic hypotheses regarding the rules by which chromatin structure is established. High-throughput sequencing has recently become the technology of choice for chromatin mapping studies, yet analysis of these experiments is still in its infancy. Here, we introduce a pipeline for analyzing deep sequencing maps of chromatin structure an  ...[more]

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