Dataset Information


Murine M7 leukemia derived from retroviral insertional mutagenesis of Gata1s fetal progenitors

ABSTRACT: The goal of this study is to derive a mouse model of human Down Syndrome (DS) megakaryocytic leukemia involving mutations in the hematopoietic transcription factor, GATA1 (called GATA1s mutation). We achieved this through transduction of Gata1s mutant fetal progenitors by MSCV-based retrovirus expressing a GFP marker, followed by in vitro selection (for immortalized cell lines), and then in vivo selection (for transformed cell lines) through transplantation. Here we report one such cell line [T6(6)] that gives rise to megakaryocytic leukemia (M7 leukemia) upon transplantation. Since the leukemic cells were retrovirally tagged with a GFP reporter, we sorted GFP+ leukemic blasts and generated their expression profiles by microarray.

ORGANISM(S): Mus musculus  

SUBMITTER: Stuart Orkin   Zhe Li 

PROVIDER: E-GEOD-16682 | ArrayExpress | 2010-08-01



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Developmental stage-specific interplay of GATA1 and IGF signaling in fetal megakaryopoiesis and leukemogenesis.

Klusmann Jan-Henning JH   Godinho Frank J FJ   Heitmann Kirsten K   Maroz Aliaksandra A   Koch Mia Lee ML   Reinhardt Dirk D   Orkin Stuart H SH   Li Zhe Z  

Genes & development 20100801 15

Oncogene-mediated transformation of hematopoietic cells has been studied extensively, but little is known about the molecular basis for restriction of oncogenes to certain target cells and differential cellular context-specific requirements for oncogenic transformation between infant and adult leukemias. Understanding cell type-specific interplay of signaling pathways and oncogenes is essential for developing targeted cancer therapies. Here, we address the vexing issue of how developmental restr  ...[more]

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