Transcriptomics

Dataset Information

0

Evidence for a rheostat that determines neuronal survival after traumatic brain injury


ABSTRACT: To investigate the determinants of neuronal survival after traumatic brain injury, we compared the transcriptional profiles of dying (Fluoro-Jade-positive) and immediately adjacent surviving (Fluoro-Jade-negative) neurons from the CA3 subfield of the rat hippocampus 24 hours after experimental TBI. We found that hippocampal neurons that survive TBI invariably express high levels of genes that have cellular functions involved in survival, regeneration, development, proliferation, neuronal plasticity such as cAMP response element binding protein (CREB), brain-derived-neurotrophic factor (BDNF) and mitogen-activated protein kinase 1 (MAPK1). Dying neurons express high levels of genes involved in aberrant cell cycle progression, immune response, inflammation, oxidative stress and apoptosis such as Interleukin-1β (IL-1β), caspase 3 and B-cell linker (BLNK). We conclude that shifting the balance between the global levels of these proteins with pharmacotherapeutic drugs that induce expression of cell survival associated genes, is expected to alter the cellular rheostat that determines cell survival or cell death. Replicate pooled samples (approximately 600 laser capture microdissected hippocampal neurons per sample of dying neurons (labeled with Fluoro-Jade, a fluorescent stain for degenerating CNS neurons) and surviving neurons (Fluoro-Jade-negative) were hybridized in duplicate to rat Agilent whole genome arrays.

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Helen L Hellmich   Michael Falduto 

PROVIDER: E-GEOD-16735 | ArrayExpress | 2010-12-20

SECONDARY ACCESSION(S): GSE16735PRJNA117425

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2010-12-20 | GSE16735 | GEO
2012-12-23 | E-GEOD-31357 | ArrayExpress
2015-01-08 | E-GEOD-59645 | ArrayExpress
| GSE92363 | GEO
2012-09-06 | E-MTAB-795 | ArrayExpress
2015-01-08 | E-GEOD-59646 | ArrayExpress
2015-01-08 | E-GEOD-64733 | ArrayExpress
2010-05-27 | E-GEOD-19701 | ArrayExpress
2014-10-14 | E-GEOD-61085 | ArrayExpress
2014-06-14 | E-GEOD-58483 | ArrayExpress