Transcriptomics,Multiomics

Dataset Information

37

Transcription profiling by array of human peripheral blood samples from Kawasaki disease patients after treatment with intravenous immunoglobulin and methylprednisolone


ABSTRACT: Clinical score and transcript abundance patterns identify Kawasaki disease patients who may benefit from addition of methylprednisolone. Intravenous immunoglobulin (IVIG) treatment-resistant patients are high risk of developing coronary artery lesions (CALs) with Kawasaki disease (KD). The IVIG-responsive (Group A; n = 6) and -resistant patients (Group B) were predicted before starting the initial treatment using the Egami scoring system, and randomly allocated a single-IVIG treatment group (Group B1; n = 6) or a IVIG-plus-methylprednisolone (IVMP) combined therapy group (Group B2; n = 5). We investigated transcript abundance in the leukocytes of those patients using microarray analysis. Results: five patients in Group A and 1 patient in Group B1 responded to initial IVIG treatment. All Group B2 patients responded to IVIP-plus-IVMP combined therapy. Prior to performing these treatments, those transcripts related to IVIG-resistance and to the development of CALs, such as IL1R, IL18R, oncostatin M, suppressor of cytokine signaling-3, S100A12 protein, carcinoembryonic antigen-related cell adhesion molecule-1, matrix metallopeptidase-9 and polycythemia rubra vera-1 were more abundant in Group B patients in comparison to Group A patients. Moreover, those transcripts in Group B2 patients were more profoundly and broadly suppressed than Group B1 patients after treatment. Conclusion: this study elucidated the molecular mechanism of the effectiveness of IVIG-plus-IVMP combined therapy. 34 samples of pre- and post-treatment in three groups consisting of predicted as IVIG-responsive patients, given single-IVIG treatment patients and IVIG-plus-IVMP combined therapy group in predicted as IVIG-resistant patients.

REANALYSED by: E-GEOD-16797

ORGANISM(S): Homo sapiens  

SUBMITTER: Yayoi Nakahata  Kenichi Kokubo   Kayoko Sato   Kazumasa Akiyama   Hirosuke Kobayashi   Masahiro Ishii   Keiko Nomoto   Shohei Ogata   Kastunori Minoura   Yoshihito Ogihara    

PROVIDER: E-GEOD-16797 | ArrayExpress| 2015-09-04

SECONDARY ACCESSION(S): GSE16797PRJNA117505

REPOSITORIES: GEO, ArrayExpress

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Publications

Clinical score and transcript abundance patterns identify Kawasaki disease patients who may benefit from addition of methylprednisolone.

Ogata Shohei S   Ogihara Yoshihito Y   Nomoto Keiko K   Akiyama Kazumasa K   Nakahata Yayoi Y   Sato Kayoko K   Minoura Katsunori K   Kokubo Kenichi K   Kobayashi Hirosuke H   Ishii Masahiro M  

Pediatric research 20091101 5


Intravenous immunoglobulin (IVIG) treatment-resistant patients are high risk of developing coronary artery lesions with Kawasaki disease. The IVIG-responsive (Group A; n = 6) and IVIG-resistant patients (Group B) were predicted before starting the initial treatment using the Egami scoring system and randomly allocated as a single-IVIG treatment group (group B1; n = 6) or as a IVIG-plus-methylprednisolone (IVMP) combined therapy group (group B2; n = 5). We investigated the transcript abundance in  ...[more]

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