Transcriptomics

Dataset Information

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Expression profiling of mouse glioma-initiating cell-like cells (GICs) and non-GICs


ABSTRACT: To identify factors involved in glioma-initiating cells (GICs), we compared gene expression between GIC-like cells and non-GICs. Neural stem cells (NSCs) were transfected with pCMS-EGFP-HRasL61 and pBabe-neo by electroporation and cultured in 0.5 mg/ml neomycin. The GFP-positive stable NSCs (NSCL61s) were purified by flow cytometry. Total RNA was prepared using RNeasy Mini Kit (QIAGEN).

ORGANISM(S): Mus musculus  

SUBMITTER: Toru Kondo  

PROVIDER: E-GEOD-17062 | ArrayExpress | 2009-11-30

SECONDARY ACCESSION(S): GSE17062PRJNA123399

REPOSITORIES: GEO, ArrayExpress

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Publications

Sox11 prevents tumorigenesis of glioma-initiating cells by inducing neuronal differentiation.

Hide Takuichiro T   Takezaki Tatsuya T   Nakatani Yuka Y   Nakamura Hideo H   Kuratsu Jun-ichi J   Kondo Toru T  

Cancer research 20091006 20


Recent findings have shown that malignant tumors contain cancer-initiating cells (CIC), which self-renew and are tumorigenic. However, CICs have not been characterized properly due to lack of specific markers. We recently established a mouse glioma cell line, NSCL61, by overexpressing an oncogenic HRas(L61) in p53-deficient neural stem cells. Using limiting dilution assays, we show that only 2 of 24 NSCL61 clones retained their tumorigenicity in vivo, although the others also expressed oncogenic  ...[more]

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