Transcriptomics

Dataset Information

33

Gene expression signatures of symptomatic respiratory viral infection in adults


ABSTRACT: Diagnosis of acute respiratory viral infection is currently based on clinical symptoms and pathogen detection. Use of host peripheral blood gene expression data to classify individuals with viral respiratory infection represents a novel means of infection diagnosis. We used microarrays to capture peripheral blood gene expression at baseline and time of peak symptoms in healthy volunteers infected intranasally with influenza A H3N2, respiratory syncytial virus or rhinovirus. We determined groups of coexpressed genes that accurately classified symptomatic versus asymptomatic individuals. We experimentally inoculated healthy volunteers with intranasal influenza, respiratory syncytial virus or rhinovirus. Symptoms were documented and peripheral blood samples drawn into PAXgene tubes for RNA isolation.

REANALYSED by: E-GEOD-17156

ORGANISM(S): Homo sapiens  

SUBMITTER: Lawrence Carin   Geoffrey S Ginsburg 

PROVIDER: E-GEOD-17156 | ArrayExpress | 2009-08-06

SECONDARY ACCESSION(S): GSE17156PRJNA119759

REPOSITORIES: GEO, ArrayExpress

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Publications


Acute respiratory infections (ARIs) are a common reason for seeking medical attention, and the threat of pandemic influenza will likely add to these numbers. Using human viral challenge studies with live rhinovirus, respiratory syncytial virus, and influenza A, we developed peripheral blood gene expression signatures that distinguish individuals with symptomatic ARIs from uninfected individuals with >95% accuracy. We validated this "acute respiratory viral" signature-encompassing genes with a kn  ...[more]

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