Transcriptomics

Dataset Information

3

Gene expression in LCMV-specific Blimp-1 deficient effector CD8+ T cells compared to wildtype effector CD8+ T cells


ABSTRACT: Antigen-specific effector CD8+ T cells deficient in Blimp-1 (Prdm1) do not acquire maximal effector functions, evade terminal differentiation, and more rapidly acquire some hallmark properties of memory CD8+ T cells. In this study, we compared the gene expression profiles of wildtype and Prdm1-/- LCMV-specific effector CD8+ T cells to better understand the molecular mechanisms underlying this striking phenotype. DNA microarray analysis was performed of DbGP33-41 and DbNP396-404 tetramer-positive effector CD8+ T cells FACS-sorted at day 8 post-LCMV infection from four independent samples of either Blimp-1 conditional knockout mice (CKO; Blimp-1flox/flox x GranzymeB-cre+) or wildtype (WT) littermate controls.

ORGANISM(S): Mus musculus  

SUBMITTER: Anmol Chandele  Rachel L Rutishauser  Kathryn Calame  Gislaine A Martins  Ian A Parish  Sergey Kalachikov  Joshy Jacob  Rachel Rutishauser  Susan M Kaech 

PROVIDER: E-GEOD-17211 | ArrayExpress | 2010-08-19

SECONDARY ACCESSION(S): GSE17211PRJNA119707

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptional repressor Blimp-1 promotes CD8(+) T cell terminal differentiation and represses the acquisition of central memory T cell properties.

Rutishauser Rachel L RL   Martins Gislâine A GA   Kalachikov Sergey S   Chandele Anmol A   Parish Ian A IA   Meffre Eric E   Jacob Joshy J   Calame Kathryn K   Kaech Susan M SM  

Immunity 20090806 2


During acute infections, a small population of effector CD8(+) T cells evades terminal differentiation and survives as long-lived memory T cells. We demonstrate that the transcriptional repressor Blimp-1 enhanced the formation of terminally differentiated CD8(+) T cells during lymphocytic choriomeningitis virus (LCMV) infection, and Blimp-1 deficiency promoted the acquisition of memory cell properties by effector cells. Blimp-1 expression was preferentially increased in terminally differentiated  ...[more]

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